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KMID : 1377020150120040231
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Tissue Engineering and Regenerative Medicine
2015 Volume.12 No. 4 p.231 ~ p.238
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Effect of type I collagen derived from tilapia scale on odontoblast-like cells
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Jia Tang
Takashi Saito
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Abstract
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Type I collagen is the most abundant extracellular matrix in bone and dentin. It was reported to be effective in inducing the osteoblast differentiation. Majority of the type I collagen used in scientific research are from bovine skin, porcine skin and rat tail. Recently, a novel type I collagen possessing sound thermal stability, was isolated from tilapia fish scale. It was reported to be able to induce the differentiation of human mesenchymal stem cells into osteoblasts. However, no information is available regarding its effect on odontoblast-like cells. The purpose of this study was to investigate the effect of type I collagen derived from tilapia fish scale on odontoblast-like cells. Type I collagen was immobilized to tissue culture polystyrene dish. Biocompatibility of type I collagen was evaluated in terms of initial cell number, alkaline phosphatase activity (ALP activity), odontogenic gene expression and calcific deposition. The results showed that type I collagen purified from tilapia fish scale significantly enhanced the cell attachment to nearly twofold as compared to control; moreover, cells cultured in type I collagen-modified substrate was induced to differentiate toward odontoblast lineage as demonstrated by upregulation of ALP activity on day 7, enhancement of ALP, BSP mRNA expression on day 7 and 10, and accelerated mineralization on day 9. It is therefore suggested that type I collagen derived from tilapia scale, an underutilized resource, holds promise as scaffolding material in the application of tissue engineering in dental field.
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KEYWORD
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Type I collagen, Tilapia scale, Mouse dental papillae cells, Odontoblast, Cell differentiation
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